《植物生理学报》 2015, 51(12): 2143-2151

茶树中两个HSF转录因子基因分离与温度胁迫响应的比较分析

熊洋洋², 顾雅琦², 刘志薇¹, 李彤¹, 吴致君¹, 庄静^1,*

南京农业大学¹园艺学院, 茶叶科学研究所, ²生命科学学院, 南京210095

通信作者：庄静；E-mail: zhuangjing@njau.edu.cn；Tel: 025-84395182

摘　要：

以茶树品种‘迎霜’为实验材料, 从中分离获得2个编码HSF转录因子的基因。进化分析显示, 这2个转录因子分别属于HSF转录因子家族的A5和A4亚族, 分别命名为CsHsfA5和CsHsfA4。序列分析显示, CsHsfA5和CsHsfA4的开放阅读框分别为1 440和1 224 bp, 分别编码479和407个氨基酸, 均含有HSF转录因子保守结合域。CsHsfA5和CsHsfA4均属于亲水性蛋白, 具有3个α-螺旋和4个β-折叠的三级结构。实时定量PCR表明, CsHsfA5和CsHsfA4基因在不同温度胁迫下均能在3个不同茶树品种‘迎霜’、‘安吉白茶’和‘云南十里香’中诱导表达, 且表达量呈现差异。

关键词：茶树; HSF转录因子; 进化分析; 温度胁迫; 基因表达分析

收稿：2015-10-08   修定：2015-11-05

资助：国家自然科学基金(31200520和31570691)和国家大学生创新创业训练计划(201410307030)。

Isolation and Expression Profiles Analysis of Two HSF Transcription Factor Genes under Temperature Stress in Camellia sinensis

XIONG Yang-Yang², GU Ya-Qi², LIU Zhi-Wei¹, LI Tong¹, WU Zhi-Jun¹, ZHUANG Jing^1,*

¹Tea Research Institute, College of Horticulture, ²College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: ZHUANG Jing; E-mail: zhuangjing@njau.edu.cn; Tel: 025-84395182

Abstract:

In this study, two genes, encoding heat shock factor (HSF), were cloned from tea plant cultivar ‘Yingshuang’. Phylogenetic analysis showed that the two HSFs respectively belong to A5 and A4 subfamily of HSF transcription factor family, and then named them as CsHsfA5 and CsHsfA4, respectively. The full-length of the open reading frame of CsHsfA5 was 1 440 bp, which encoded 479 amino acids. The full-length of the open reading frame of CsHsfA4 was 1 224 bp, which encoded 407 amino acids. The CsHsfA5 and CsHsfA4 were hydrophilic proteins. The three-dimensional structures of CsHsfA5 and CsHsfA4 were identical in the DNA binding domain, which had 3 α-helix and 4 β-strands. Quantitative real-time PCR analysis of the expression profiles showed that the CsHsfA5 and CsHsfA4 genes could be induced by low or high temperature treatments. The expression levels of CsHsfA5 and CsHsfA4 genes exhibited difference among three tea plant cultivars ‘Yingshuang’, ‘Anjibaicha’, and ‘Yunnanshilixiang’.

Key words: Camellia sinensis; HSF transcription factor; phylogenetic analysis; temperature stress; gene expression profiles